This is particularly true for those protein preparations that are typically low in yield or less stable in solution, like membrane receptor systems.
Since we observed fast and marked inhibitory effects of Ro5-4864 treatment on various signaling events and effector functions in MCs stimulated via different plasma membrane receptor systems, we sought to address the localization of TSPO in MCs by means of heterologous expression of a fluorescent TSPO fusion protein and confocal microscopy.
Of note is that PDT causes fundamental changes to membrane receptor systems and the signal-transducing capabilities of the target cells, some of which are associated with growth suppression (e.g., by OSM) or growth stimulation (e.g., by EGF) of Ep (Wong et al, 2003; Liu et al, 2004).
We also tested the cell response to glutamic acid (L-glu) and ATP which, like other physiological extracellular signals, induce [Ca2+]i variation in astroglial cells via membrane-receptor systems.
AI-2 is taken up by a specific membrane receptor transport system.
Within the immune system, membrane receptor clustering by multivalent antigen binding is commonly the first stage in signal transduction and the consequent cellular response to allergen exposure.
In more complex systems, membrane receptor proteins sense environmental changes and trigger a cascade of intracellular molecular events involving "second messengers" or protein modifcation cascades that lead to concerted changes in the expression of several genes.
In this system a membrane receptor acts upon an enzyme, phospholipase C, located on the inner surface of the cell membrane.
Here we demonstrate and characterize a model system for membrane receptor investigations using SEIRA spectroscopy.
RANK is a membrane receptor on osteoclasts and immune system cells, and binding with RANKL is necessary to promote differentiation, survival and activation of osteoclastic cells.
