Sentence examples for membrane protein sample from inspiring English sources

The major bottleneck in obtaining membrane protein structures is the notorious difficulty involved in expressing and purifying the large quantities of membrane protein sample required for X-ray crystallography.

These steps were repeated six to seven times to extract the red blood cell white ghost, which was used as a red blood cell membrane protein sample for subsequent examinations.

The supernatant was mixed with an equal volume of membrane protein sample buffer (10% SDS, 3% β-mercaptoethanol, 15% glycerol, 0.025% bromophenol blue and 75 mM Tris/HCl, pH 7.0) and incubated at 40°C for 30 min.

High amounts of membrane protein samples are needed for structural or functional analysis and a first bottleneck is often to obtain sufficient production efficiencies.

The efficiency and robustness of lysates from Escherichia coli strains allow a wide diversity of applications and we summarize current strategies for the successful production of high quality membrane protein samples.

Unlike for samples of soluble proteins, which need be boiled before loading, membrane protein samples cannot be boiled since boiling results in further aggregation.

Therefore, membrane protein samples cannot be completely denatured only by SDS, thus have a more compact shape, which tends to faster migration in the SDS-PAGE gel, typically at ∼70 85% of their expected molecular weight [57].

Membrane protein samples were supplemented with 0.1% DDM.

Arabidopsis membrane protein samples were prepared as described in [ 9].

The data used were derived from Arabidopsis membrane protein samples labeled with iTRAQ reagents.

However, PIEF and OGE surprisingly provided very different results when applied to iTRAQ-labeled membrane protein samples (see below).