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The novel concept of membrane protein crystallization from lipidic cubic phases, "in cubo", has yielded well-ordered crystals and high-resolution structures of several membrane proteins, yet progress has been slow due to the lack of understanding of the molecular mechanisms of protein transport, crystal nucleation, growth, and defect formation in cubo.
Borshchevskiy, V. et al. Isoprenoid-chained lipid β-XylOC16+4 A novel molecule for in meso membrane protein crystallization.
Salvati Manni, L. et al. Phase behavior of a designed cyclopropyl analogue of monoolein: implications for low-temperature membrane protein crystallization.
Hato, M., Hosaka, T., Tanabe, H., Kitsunai, T. & Yokoyama, S. A new manual dispensing system for in meso membrane protein crystallization with using a stepping motor-based dispenser.
This chapter reviews those microfluidic crystallization methodologies that have proven their utility for membrane protein crystallization.
We are utilizing a variety of techniques for the development and implementation of detergent-specific membrane protein crystallization screens.
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Although recent reports on membrane protein crystallizations in microfluidic devices are a relatively new addition to the arsenal of tools available to membrane protein crystallographers, they are promising.
Despite the advent of many innovative crystallization approaches (see Section 3) the growth of well-ordered membrane protein crystals is still a major problem.
From this analysis we identified the most successful conditions and applied them in the design of new sparse and incomplete factorial matrices to screen membrane protein 2D crystallization.
Six membrane proteins have yielded diffracting 2D crystals suitable for structure determination, indicating that these new matrices show promise to accelerate the success rate of membrane protein 2D crystallization.
Their influence ranges from domain formation in intact biomembranes to membrane protein reconstitution and crystallization.
More suggestions(15)
membrane protein isolation
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membrane protein purification
membrane distillation crystallization
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membrane protein preparation
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membrane protein quality
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