Sentence examples for membrane protein Extraction from inspiring English sources
Plasma membrane fractionation was performed using a membrane protein extraction kit from BioVision.
Cell membranes and cytosolic fractions were freshly prepared from cells by using a Membrane Protein Extraction Kit (Thermofisher, USA).
For membrane extraction, fresh tissue samples were used according to the manufacturer's protocol (Plasma Membrane Protein Extraction Kit, BioVision, Mountain View, USA).
In the experiment described here, following a procedure reported for membrane protein extraction, the lysate was used to separate the soluble fraction, expected to contain PARPSso interacting with DNA, from the insoluble one, corresponding to membranes [19].
Total cell lysate was subjected to membrane protein extraction using Mem-PER Eukaryotic Membrane Protein Extraction Reagent kit (Pierce).
The latter allows the modulation of the hydrophobic properties of the molecule to optimize the step of membrane protein extraction.
In this work, we developed new fluorinated surfactants that exhibit detergency in terms of both lipid-vesicle solubilization and membrane-protein extraction.
DDM/DM can be applied for most eukaryotic membrane proteins in extraction step.
It is now a complementary technique to X-ray crystallography and electron microscopy for structure determination of individual membrane proteins after extraction, purification and reconstitution into lipid bilayers.
The ProteoJET™ membrane protein and cytoplasmic/nuclear protein extraction kits (Fermentas GmbH, St. Leon-Rot, Germany) were used to isolate membrane, cytoplasmic and nuclear proteins.
Total membrane proteins were extracted following the protocol of the Membrane and Cytosol Protein Extraction Kit.
