Sentence examples for membrane probed with from inspiring English sources

A replicated membrane probed with the secondary antibody alone did not display signals.

Proteins were separated by 14% SDS-PAGE, blotted onto PVDF membrane, probed with the primary Ab followed by secondary Ab-HRP.

The protein was transferred to a PVDF membrane, probed with anti-PW1 and anti-Hsp70 antibody, and developed using an ECL chemiluminescence kit (Pierce).

The proteins were transferred onto the nitrocellulose membrane, probed with appropriate primary antibodies and HRP-conjugated secondary antibodies, and were visualized by the enhanced chemiluminescence (VWR, West Chester, PA).

The hybridization signal strength for each cDNA clone on membrane probed with forward subtracted cDNA was compared with cDNA clones on the duplicate membrane probed with reverse subtracted cDNA.

The samples were subjected to SDS-PAGE and proteins were electrotransferred onto a polyvinylidene difluoride membrane, probed with appropriate antibodies and detected as described previously [ 21].

Membranes probed with pre-immune serum served as a negative control.

There were no visible bands detected on membranes probed with pre-immune serum and processed identically (data not shown).

Protein-matched whole cell lysates were separated by SDS-PAGE, transferred to nitrocellulose membranes, probed with the antibody of interest and developed with enhanced chemiluminescence (ECL).

Subsequently proteins were blotted and membranes probed with anti-c-Raf1 or anti-B-Raf.

For WB analysis, proteins were transferred onto PVDF membranes, probed with antibodies, and analysed using the LAS-3000 System (Fujifilm).