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Mitochondrial membrane potential was evaluated by Rhodamine 123 staining.
The resting membrane potential was taken 3 min after a stable recording was first obtained.
The membrane potential was held at the existing resting membrane potential during the current injection.
Golgi membrane potential was therefore assumed to be small or nonexistent.
Before calcium entry, a partial breakdown of the membrane potential was observed in both polar regions.
In addition, a change in the mitochondrial membrane potential was clearly observed in cells treated with either Pcs.
Effect of sub MIC levels of PtNPs on membrane potential was discerned using fluorophore DiSc3 as reported earlier70.
Mitochondrial membrane potential was evaluated by staining with rhodamine 123.
Mitochondrial membrane potential was measured according to the manufacturer's instructions.
In all the cases, the membrane potential was registered.
The mitochondrial membrane potential was measured by flow cytometry using JC-1 dye.
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