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The study of the mitochondrial membrane potential using Mito-Tracker dye showed that NO decrease the mitochondrial function.
Fluorescent protein voltage sensors are recombinant proteins that are designed as genetically encoded cellular probes of membrane potential using mechanisms of voltage-dependent modulation of fluorescence.
To exclude this possibility, we eliminated membrane potential using high potassium bathing solution (133 mM KCl).
We next studied the effects of HKIII overexpression on membrane potential using TMRE as previously described [27].
We estimated mitochondrial membrane potential using the positively charged fluorescent probe tetramethylrhodamine ethyl ester (TMRE) in xav mutant and WT embryos at ∼56 hpf.
Spontaneous responses of multiple pyramidal cells (PCs) were simultaneously recorded at resting membrane potential using whole-cell patch-clamp technique in the medial PFC of young and adult rats (P16 P30, n = 108; 3–12 months, n = 8).
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In addition, mitochondrial function as a measure of aging was assessed by determining mitochondrial membrane potentials using flow cytometry and by amplifying mitochondrial DNA deletions associated with aging.
One particularly daunting problem has been the recording of neuronal membrane potentials using voltage-sensitive dyes [11].
Analysis of these membrane potentials using the Goldman-Hodgkin-Katz equation [30] confirmed the assumption of small anion selectivity of the Delta toxin channel and small cation selectivity of Beta toxin.
In addition, we examined mitochondrial membrane potentials using flow cytometry, as well as signaling mechanisms with an immunoblotting analysis.
During preliminary recordings, we found that inside-out patches held at more negative or positive membrane potentials using the same symmetrical Cl– solutions used for macroscopic current recordings were unstable over these prolonged recording periods.
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