The osajin effect on mitochondrial membrane potential was studied by flow cytometry after staining with MitoCapture™, a cationic dye.
To determine whether binaphthoquinones affect the mitochondrial membrane potential, we studied the ability of BiQs to depolarize mitochondrial membrane potential in both dextrose and glycerol-containing media.
The mitochondrial inner membrane potential was studied from the uptake of the cationic carbocyanine dye, JC-1 into the mitochondrial matrix.
The structural changes on the membrane potential were studied by using L-anilino naphthalene-8-sulphonate as the fluorescent probe by the method of Verma et al. [ 18] and Robertson and Rottenberg [ 19].
Because little is known about the effects of LPI as a possible vascular signalling mediator on endothelial membrane potential, this study was designed to investigate the effects of LPI on intracellular Ca2+ concentration, membrane potential, and to explore the underlying ion conductance in endothelial cells.
To elucidate the mechanism of cell death induced by quercetin, we chose to measure the mitochondrial membrane potential because previous studies have shown that the single mitochondrion of the kinetoplastid parasite is a good indicator of cellular dysfunction [16], [19], [33].
Regardless, it would be useful to investigate mtDNA alteration on ischemic retina using measurements for 8-hydroxy-2-deoxyguanosine and mitochondrial membrane potential, and future studies need to address this more detail.
Neuroscientists make use of measures of correlation between firing rates, rather than between membrane potentials, to study cross-neuron communication.
Changes in mitochondrial membrane potential (ΔΨm) were studied by staining the cells with the cationic dye, JC-1, according to the manufacturer's instructions (Molecular Probes, Eugene, OR USA) as described previously.
