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The criteria for the acceptation of each experiment also included less than 5% changes in resting membrane potential, spike magnitude, and input/seal resistance.
The criteria for the acceptation of each experiment also included less than 5% changes in resting membrane potential, spike magnitude, input/seal resistance throughout each of experiments.
The criteria for the acceptation of each experiment also included less than 5% changes in resting membrane potential, spike magnitude, input resistance and seal resistance throughout each of experiments.
With current injection at the soma, in the absence of biochemical signaling, there is a significant calcium transient at the level of the spines, corresponding to that first membrane potential spike.
Irrespective of age or metabolic phenotype, subsequent electrophysiological recordings from POMCp110β null neurons demonstrated that there were no alterations to POMC neuron resting membrane potential, spike firing frequency, or input resistance in comparison to littermate control POMC neurons (Table S2).
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Interestingly, the calcium obtained from CF stimulus superimposed with current injection results in earlier membrane potential spiking than the supralinear calcium release from PF stimulus and current injection superposition.
The amplitude of the graded component of the presynaptic calcium signal can vary in time, and small fluctuations in resting membrane potential alter spike frequency and even switch spiking on and off.
Understanding the "translation" of synaptically driven fluctuations of membrane potential to spike events is of key importance to our understanding of computational processes in the brain.
Recent experimental demonstrations of critical slowing down as the membrane potential approaches spike threshold demonstrate critical dynamics to profoundly affect the AP generation (Meisel et al., 2015).
Injection of a small hyperpolarizing current to prevent the membrane potential crossing spike threshold revealed the underlying generator signal as a transient depolarization just after light onset and a transient hyperpolarization at light offset (blue trace).
However, these neurons had lower resting membrane potential and spike firing frequency than control POMC neurons, which may imply a reduction in basal transmitter release, which could alter peripheral glucose homeostasis.
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