The phenomenon of membrane potential-sensitivity signaling has been observed in many GPCRs, including the M2 receptor (Ben-Chaim et al. 2003), P2Y1 (Gurung et al. 2008), the α2A-adrenoceptor (Rinne et al. 2013), the β1-adrenoceptor (Birk et al. 2015), the dopamine D2 receptor (Sahlholm et al. 2008), and the histamine H3 receptor (Sahlholm et al. 2012).
This is important because cell membrane fluidity influences membrane-bound receptor functioning, thereby signal transduction, ion transport, membrane potential and receptor sensitivity (45).
We did not measure membrane potential, but the sensitivity of NO- and prostaglandin-independent relaxation to TRAM34 and apamin suggests that this effect was mediated via an EDH-like pathway.
Taken together, our results indicate that PCB potentially influences acid-sensitivity through alteration of the membrane potential of acid-sensitive neurons, which could affect the regulation of respiration.
A third missense variant, V123L, in a patient with lone AF, reduced resting membrane potential and altered pH sensitivity in patch-clamp experiments, with structural modeling predicting instability in the vicinity of the TASK-1 pore.
A high sensitivity of membrane potential to the ratio of ion diffusion coefficients is demonstrated.
SMF sensitivity of membrane potential was higher for normal cells, when they were challenged by NPs and reverse otherwise.
In line with this mechanism, we found that mouse K2P2.1 (TREK-1) carrying a mutation in Ct at a key phosphorylation site, S300A (Murbartian et al, 2005), displayed decreased sensitivity to membrane potential changes.
In particular, the four IC7-1 derivatives possessing longer alkyl chains (IC7-1-Pr to -He) displayed higher sensitivity to membrane potential than the derivatives with shorter alkyl chains (IC7-1-Me and -Et).
