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In CDKAL1 KO cells, the glucose-stimulated mitochondrial membrane potential response was blunted (Fig. 7B).
We hypothesized that altered mitochondrial glucokinase activity contributes to the poor membrane potential response to glucose stimulation.
CDKAL1 KO cells exhibited a significantly reduced membrane potential response to glucose stimulation compared with WT cells (Fig. 7B).
A potential cause of the decreased mitochondrial membrane potential response to glucose is altered mitochondrial mass [30].
The membrane potential response is also dependent on the neuron's morphology, the distribution of intrinsic ion channels and the effective localization of ChRGR.
In addition, KIC, which is a direct substrate for the TCA cycle, bypassing glycolysis, also reduced the mitochondrial membrane potential response (Fig. 7C).
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In this study, we explored the feasibility of bioengineered primary olfactory sensory neurons (OSNs) as sensing elements of biomimetic olfactory-based biosensors, in which light addressable potentiometric sensor (LAPS) was used to monitor bioengineered OSNs membrane potential responses to odorant molecules.
The model predicts that ICcl responses are more multiplicative in spike count responses than in membrane potential responses.
Experimentally observed membrane potential responses to ITD and ILD could be produced in model ICx neurons using a linear combination of ICcl spiking responses (Figure 6).
Regular-spiking neurons were selected by their pyramidal appearance and their membrane potential responses to constant step current stimuli (Fig. 1A).
Representative membrane potential responses to the current protocol composed of alternating negative and positive current pulses (−50 pA, +50 pA) are shown in figure 3G, H.
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