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These were delivered in the narrow membrane potential range in which Ih and outward currents were not significantly activated (−70 to −75 mV).
As the model was used for simulations within a very narrow membrane potential range, it was simplified to not include any active membrane conductances.
We then investigated the action of serotonin on the currents active in the subthreshold membrane potential range.
In the membrane potential range from −65 to −40 mV, the curve is almost linear with a conductance of 0.5 0.6 nS/pF.
In the physiological membrane potential range, it is highly similar to our Boltzmann fit of Figure 1(c), which appears in Figures 4(a) and 4(c) as a dark gray dashed curve.
This asymmetrical change in voltage response occurs for membrane resting potentials higher than −55 mV because h-channel is mostly activated in the hyperpolarized membrane potential range and has a smaller effect on resonant properties than Ca2+- (CaS- and CaT-) channels (in association with KCa-channel) in this range.
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The first striking feature one observes is that in case of normal cells there is a much wider distribution of membrane potential ranging from 150 270 mV, the corresponding range in cancer cells being 200 250 mV.
As shown in Figure 7 d), the resonant behavior depends on two membrane potential ranges.
Resting membrane potential ranged from −30 mV to −66 mV (mean −45±± 8.60 mV, n = 40).
At membrane potentials ranging from −100 mV to +40 mV, inward currents were never detected.
NMDA current-voltage relationships revealed that co-applied DHPG inhibited NMDA currents only at membrane potentials ranging from −100 to −40 mV, in neurons expressing Homer1a, but not in control neurons (Fig. 3A).
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