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Mitochondrial membrane potential provides a valuable indicator of cells' health and functional status.
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Measurements of membrane potential provide useful additional information.
Healthy mitochondria have a high mitochondrial membrane potential that provides the electrochemical energy to drive ATP synthesis through oxidative phosphorylation [18].
Recent advances in genetically engineered calcium and membrane potential indicators provide the potential to estimate the activation dynamics of individual neurons within larger, mesoscale networks (100s 1000 + neurons).
Disturbances in the membrane potential can provide a rapid and sensitive indication of those stimuli that lead to physiological functionally important changes with respect to bacterial viability [ 1].
It is thought that C. elegans neurons are generally isopotential (Goodman et al. 1998), so membrane potential could provide the long-range signal necessary to communicate from one Ca2+ compartment to another.
The experimental protocols for Western blots, Ras activity measurements cell growth and soft agar assays, LDH activity measurements, and for measuring mitochondrial mass and membrane potential are provided in the Additional file 2. The authors declare that they have no competing interests.
While imaging is a useful technique for observing mitochondrial membrane potential heterogeneity and provides the advantage of observations of mitochondrial morphology, this technique is not suitable for measurement of electrophoretic mobility, which can provide information about surface properties of mitochondria which could be used to separate subpopulations.
Because rhod123 is cationic, it selectively accumulates in the mitochondria matrix proportional to the electronegativity of the mitochondrial membrane potential and thus provides a quantitative measure of ΔΨm.
The OHC electromotility is supported by a somatic motor in the OHCs body, which has the ability to elongate or contract axially, due to changes in membrane potential, and could provide positive feedback to reduce viscous damping and provide active amplification.
In this mock example the moving average of the membrane potential seems to provide very similar information as the spike density function.
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