Importantly, we showed that mitochondrial membrane potential and morphology are available for the phenotype analysis in DT40.
These properties are dependent on membrane potential, nuclear morphology, and cellular connectivity and vascularity that differ between normal and malignant tissue.
ATP synthases maintain mitochondrial membrane potentials and morphology [26].
The anti-apoptotic mechanism of BI-1 involves the suppression of Bax activation and translocation to mitochondria, preserving the mitochondria membrane potential and mitochondrial morphology, and preventing the activation of post-mitochondrial caspases.
Similarly, the elevated ROS levels in mitophagy-deficient cells under caloric restrictions cause mitochondrial dysfunctions such as reduced respiration, lowered membrane potential and aberrant morphologies, ultimately leading to shortened chronological life span.
With the disruption of the mitochondrial membrane potential, changes in cellular morphology appeared as a result of significant apoptosis induction.
In a previous study we could show that an acute downregulation of parkin in SH-SY5Y cells, primary mouse neurons and Drosophila S2 cells affects mitochondrial morphology, membrane potential and function [39].
In parallel experiments, the localization of the ectopically expressed p32 proteins was determined via immunostaining for the Myc epitope tag together with co-staining with MitoTrackerRed dye that accumulates in the mitochondria with intact membrane potential to define mitochondrial morphology and co-localization.
Disease-related mutations in these genes cause loss of protein function and contribute to impaired mitochondrial integrity as revealed by the loss of mitochondrial membrane potential (MMP), disrupted mitochondrial morphology and reduced activity of complex I of the electron transport chain.
Vowinckel, J., Hartl, J., Butler, R. & Ralser, M. MitoLoc: A method for the simultaneous quantification of mitochondrial network morphology and membrane potential in single cells.
