Sentence examples for membrane potential dye from inspiring English sources

10 µl of 30X mitochondrial membrane potential dye was added to each well.

DiBAC4(3) is a lipophilic and anionic bis-oxonal membrane potential dye with uptake of the dye restricted to depolarized cells or cells with disrupted cytoplasmic membranes.

For non-CNiFER cell lines, Blue Membrane Potential dye (Molecular Devices, Sunnyvale, CA) was prepared in aCSF and diluted 2 fold the recommended dilution.

For the fluorescent test with the Membrane Potential dye (Molecular Devices) the differentiated cells were incubated in 25 µl/well of the dye solubilised in Tyrode's buffer for 30 min at 37°C plus 30 min at room temperature.

Since GABA is a chloride channel and glutamate receptors permit passage of not only Ca2+ but also Na+, we analyzed the cells with the FLIPR384 using the voltage-sensitive dye (named Membrane Potential dye) able to detect non Ca2+ ionic flux into the cells.

This was centrifuged at 1000 rpm for 2 min to embed an even layer of cells, and then incubated at room temperature for 45 min in 100 µl buffer containing membrane potential dye (Blue Kit, Molecular Devices, Wokingham, UK) before being assayed in the FlexStation (Molecular Devices).

Various in vitro assays such as membrane potential dyes [ 2], rubidium efflux [ 3], radioligand binding [ 4] and patch clamp [ 5] have been used to identify compounds displaying cardiac toxicity.

We evaluated the suitability of the membrane potential dyes TMRM, R123, and JC-1 for use in isolated mitochondria since the free dye must be removed from the medium prior to CE-LIF analysis (see Figure S-2-1 of the Supporting Information).

Although recent evidence suggests GI neurons may be more prevalent, when improved slice techniques are used in conjunction with novel methods for identifying GI neurons (e.g., membrane potential dyes) as many as ∼30 40% of all neurons in the VMH are reported to be GI neurons (40).

Results in model membranes are consistent with flow cytometry experiments in Escherichia coli and Staphylococcus aureus using a membrane potential sensitive dye (bis-oxonol) and a nucleic acid dye (propidium iodide), suggesting that the mechanism of action is based on membrane binding and collapse of membrane integrity by depolarization and permeabilization.

JC-1 is a vital cationic mitochondrial dye that is lipophilic and becomes concentrated in the mitochondria in proportion to the membrane potential; more dye accumulates in mitochondria with greater Δψm and ATP generating capacity.