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The membrane potential was held at the existing resting membrane potential during the current injection.
LPS-transfected GSDMD-/ macrophages also maintained their mitochondrial polarization during the imaging timeframe, unlike LPS-transfected wild-type macrophages that lost their mitochondrial membrane potential during pyroptosis induction (Fig. 8b, e, f, Supplemental Fig. 14b and Supplemental Movie 19).
SK3 channels are voltage-independent Ca2 +-dependent K+ channels that play a key role in regulating smooth muscle membrane potential during purinergic inhibitory neurotransmission in the colon.
The uncertain dynamics of the membrane potential during neuronal discharge is shown in Fig. 1(a).
The time curse of the membrane potential during the spike is described by differential equations, like Hodgkin-Huxley's [35].
This equation gives the variation in membrane potential during a period of rest (no spike) of the neuron.
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After the desired time of incubation at 37 °C, efflux was stopped by the addition of ice-cold stop solution and vesicle associated radioactivity was measured as described in 2.5.1 Membrane potentials during efflux studies were generated as described before (section 2.5.1.1).
There is evidence that mitochondrial respiratory complex activity and mitochondrial membrane potential decline during aging and that endogenous antioxidant system activities exhibit an age-dependent decrease, making redox imbalance play a more predominant role in the aged cells and tissues.
Here we introduce a new, automatic and fast method to map realistic neurons into equivalent reduced models running up to >40 times faster while maintaining a very high accuracy of the membrane potential dynamics during synaptic inputs, and a direct link with experimental observables.
Macrophage membrane potential depolarized during the first 12 h post-infection, which associated with a decreased inward potassium current density, changed in inward rectifier conductance and increased outward potassium current density.
But this requires to replace the firing condition (4) by another condition stating what the membrane potential does during the spike.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com