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After incubation, the cells were washed with PBS once, and the mitochondrial membrane potential was detected by using the JC-1 mitochondrial membrane potential detection kit, according the instructions of the manufacturer (Biotium, Hayward, CA, USA).
Mitochondrial membrane potential detection kit was obtained from Cell Technology, Inc. (Mountain View, CA).
The change in mitochondrial membrane potential in the astrocytes was monitored using the mitochondrial membrane potential detection kit (Cayman Chemical Company) according to the manufacturer's instructions.
Mitochondrial membrane potential detection kits were obtained from BD Biosciences (San Jose, CA, USA).
The mitochondrial membrane potential was analyzed using the JC-1 mitochondrial membrane potential detection kit (Biotium).
JC-1 Mitochondrial Membrane Potential Detection kit was used to measure mitochondrial membrane potential changes in cells (Biotium, Hayward, CA).
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Moreover, we confirmed this effect of rhArg using a mitochondrial membrane potentials detection kit (JC-1).
Mitochondrial Membrane Potential was detected using the MitoPT™ Kit (Mitochondrial Permeability Transition Detection Kit, Immunochemistry Technologies, LLC) incorporating the JC-1 cationic dye.
TMRE, a cell-permeant, cationic, red-orange fluorescent dye sequestered by active mitochondria and used to quantify mitochondrial membrane potential, has an intermediate detection range (Fig. 1B ).
Custom MATLAB software with xPCtarget toolbox (by KH) (Mathworks, MA) was used for recording the microphone signal and membrane potential data, real-time detection of the target syllable, and generation of distorted auditory feedback [DAF].
Detection of mitochondrial membrane potential (ΔΨm) was performed using the JC-1 detection kit (Biotium, Hayward, California, USA) according to the manufacturer's directions.
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