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The absolute contribution to proton leak by UCP3 is shown in Figure 3(C) by interpolation at the highest membrane potential common to all curves, 160 mV.
Respiration rates (by linear interpolation between flanking points) were calculated at the highest membrane potential common to all conditions on a given day.
To compare proton leak rates at the same driving force, Figure 1(D) shows rates interpolated at 166 mV, the highest membrane potential common to all curves generated in the experiment.
To show the effect of HNE at shorter times more clearly, Figure 1(C) shows the analysis at the highest membrane potential common to the 2.5 min data points (175 mV).
To test for differences in proton conductance by ANOVA or paired Student's t tests, we calculated respiration rates (by linear interpolation between flanking points) at the highest membrane potential common to all conditions on a given day.
Respiration rates (by linear interpolation between flanking points) at the highest membrane potential common to all conditions on a given day were used to test for differences in proton conductance by ANOVA or paired Student's t tests.
Similar(54)
Specifically, in recordings made during natural slow-wave sleep, we found evidence for 1) clear correspondence between slow waves in the LFP and alternations of active and silent states in the membrane potential, with common periods of activity and silence in simultaneously recorded cells (Fig. 7 a ).
The HNE-stimulated increase in respiration was calculated at the highest common membrane potential, 169 mV.
Proton leak under these conditions at the highest common membrane potential (137 mV) is shown in Figure 1(B).
At the highest common membrane potential, 158 mV, both carboxyatractylate and bongkrekate inhibited proton conductance through UCP3.
Previous studies have indicated that decreased mitochondrial ATP or mitochondrial membrane potential loss is the common and crucial stimulus that controls OPA1 processing.
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