The lack of ability to control membrane potential during extracellular recordings, combined with the large increase in excitability caused by M1R activation, suppresses the ability to detect the increase in EPSP amplitude in extracellular recordings.
That is, we assume that in ICcl, ITD- and ILD-dependent cues within frequency channels are combined additively in the membrane potential and are transformed to a nonlinear spiking response by the spiking input-output function.
We also studied their combined effect on the mitochondrial membrane potential (ψm).
The cell death caused by both etoposide and TRAIL appears to involve apoptosis, since the combined treatment caused a loss in mitochondrial membrane potential (ΔΨm), which is closely associated with apoptosis induction.
Wherever feasible, we combined it with the analysis of mitochondrial membrane potential (ΔΨm) by cytofluorometry and with protein analysis of OXPHOS complexes by SDS-PAGE and BNE followed by specific immunodetection of structural subunits of mitochondrial respiratory complexes.
After the combined treatment with DHA and HDACi, the mitochondria membrane potential, the expressions of Mcl-1, p-ERK, Ki61, activated caspase 3, and PARP were decreased, while p53 and Bak expressions were increased.
The results showed that cells treated with the combined TPT and ATRA induced the loss of mitochondrial membrane potential compared to TPT or ATRA groups in Fig. 3a- b.
Exposure of cells to YK led to the disappearance of red fluorescence and increase in the green fluorescence in most cells combined with a significant reduction (p <0.001) in mitochondrial membrane potential.
After combined PDT, HeLa cells showed a relevant depolarization of mitochondrial membrane potential (ΔΨm).
Koshiba et al. have reported that MAVS does not combine with RIG-I-like receptors when the mitochondrial membrane potential was decreased, which hampered the antiviral signal transduction and thereby reduced the intensity of antiviral immune response [ 39].
