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However exposure of MN1 cells to TBA resulted in a stronger variation in mitochondrial membrane potential, associated with cleavage of PARP, and more apoptosis, as measured by annexin V expression.
It was found that the reticulum-like mitochondria are mostly transformed to small and dispersed fragments, because of the CCCP-induced collapse of the mitochondrial membrane potential associated with the remodeling of mitochondrial cristae and the subsequent occurrence of morphological changes in mitochondria.
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In experimental studies, loss of mitochondrial membrane potential was associated with a decrease in MIBI uptake [3].
For three calculated regions including the synaptic site (a in Fig. 1A), a point in the dendrite on the soma side (b) leading to the transverse segment and an output site assumed on a contralateral dendrite (c), the membrane potential changes associated with a unitary synaptic activation are compared between the uniformly (blue) and nonuniformly (red) distributed active conductances (Fig. 2A).
High mitochondrial membrane potential is associated with preserved mitochondrial physiology and efficiency.
Loss of mitochondrial membrane potential is associated with the release of pro-apoptotic factors including cytochrome c.
Maintenance of mitochondrial membrane potential is associated with response to desiccation stress in worms, and importantly, the survival of dopaminergic neurons in mammals.
As mitochondrial dysfunction and decrease in the mitochondrial membrane potential are associated with the induction of apoptotic cell death, flow cytometric analysis of Ery5-treated PC-3 did not show any significant change in the mitochondrial membrane potential loss even up to 24 h (Supplementary Figure S1B).
Membrane potential-associated fluorescence was detected using a FlexStation (Molecular Devices) microplate fluorimeter, recording baseline (1 min) and following automated addition of choline (20 μL) to achieve 100 nM and 10 μM final concentrations in HBSS/HEPES 1× dye.
Since changes in mitochondrial membrane potential are usually associated with increases in ROS release [31], the effects of brominated noscapine analogs on ROS release from LPS-primed human macrophages were investigated using an enhanced chemiluminescence method [22].
Compounds of EPSCs with τrise/τdecay of 0.1/1, 0.3/3 and 0.6/6 ms induced rapid transitions of the somatic membrane potential, and were associated with temporally precise spike responses with Δt<2.45 ms (mean = 1.35 ms, s.e.m. = 0.30 ms).
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