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Importantly, these punctate mito-GFP structures retained their mitochondrial membrane potential, assessed by staining with the mitochondrial potential dye TMRM (Supplementary Figure S5).
(G ) Resting membrane potential assessed under current clamp with a gramicidin-perforated patch for cell bodies (black squares) and motile cilia (green squares) before (n = 7 cell body, n = 8 motile cilia) and after (n = 7 cell body, n = 5 motile cilia) addition of flufenamic acid (FFA, 100 μM) to the bath.
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Mitochondrial membrane potential was assessed in SH-SY5Y cells with the probe JC-1 (Invitrogen).
Membrane potential was assessed by FACS analysis of TMRE staining of trypsinized cells.
Mitochondrial membrane potential was assessed with the dual wavelength fluorescent probe, JC-1.
Mitochondrial membrane potential was assessed using the MitoPT-JC1 assay kit (#924; Immunochemistry, Bloomington, MN).
Mitochondrial membrane potential was assessed simultaneously with all measurements of oxygen consumption [56].
Mitochondrial membrane potential was assessed using lipophilic cationic fluorescent probe, tetramethyl rhodamine methyl ester (TMRM) that localizes into the mitochondria as a function of membrane potential.
As there was a loss of complex IV activity after 12 days of silencing, mitochondrial membrane potential was assessed at this time point using the JC-1 probe.
The integrity of the mitochondrial membrane potential was assessed by measuring the uptake of fluorescent dye 5, 5′, 6, 6′-tetrachloro-1, 1′, 3, 3′-tetraethylbenzimidazolcarbocyanine iodide (JC-1).
The effect of antimycin A on the cell membrane potential was assessed in current clamp mode.
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