Moreover, changes in mitochondrial membrane potential affect turnover and regulation of dysfunctional mitochondria in the cell through fusion/fission and targeting for elimination by mitophagy (mitochondrial-specific autophagy).
This approach will be more specific to examine how mitochondrial calcium uptake affects the effector functions of IL6-treated cells because mitochondrial membrane potential affected by CCCP and antimycin cannot address this point.
In eukaryotic cells, essential oils can induce the depolarization of the mitochondrial membranes by decreasing the membrane potential, affecting Ca++ and other ion channels, and reducing the pH gradient, affecting (as in bacteria) the proton pump and the ATP pool [ 51].
Considering that mitochondrial membrane potential directly affects mitochondrial activity, we monitored this parameter in our experimental paradigm.
As resting membrane potential (RMP) affects MEPP amplitude, recordings were only taken from fibers with RMP more negative than −60 mV.
Many membrane proteins are asymmetric in their functionality, because they translocate ions or solutes preferentially in one direction or because their stimulant, e.g. ligand, binding partner protein, membrane potential, etc., affects the protein from one side only.
The membrane potential may affect the activation process of a GPCR in many ways.
When the mitochondrial membrane potential is affected, JC-1 returns to its green monomeric state.
Furthermore, variations of the membrane potential may affect hair bundle dynamics through the phenomenon of reverse electro-mechanical transduction [17, 18].
