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To further optimize the membrane geometries (dimensions and angles) and investigate the influence of membrane spacing and membrane piece numbers in a helical membrane module on its performance in filtering sludge suspensions in membrane bioreactor (MBR), various membrane modules were studied.
After a blocking step in 50 mM Tris HCl [pH 7.4], 150 mM NaCl, 0.05% Tween 20 (TBST) containing 5% milk, for 1 hr at RT, each membrane piece was probed with blocking buffer containing 500 µl of SaurPrP1 antiserum, at 4°C overnight.
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After electrophoresis the two membranes were stained with amido black staining solution (0.1%) and the protein-containing membrane pieces were finally excised.
Most lymphoid cells actively capture membrane pieces from antigen-presenting cells to which they are bound during antigen recognition or from target cells such as cancer cells [21] [23].
Membrane pieces (either untreated or reduced with DTT) were packed directly into a solid-state 4.0 mm NMR rotor and sealed using end caps and driving tips.
Membrane pieces of 4.5 cm² were immersed in LB medium containing kanamycin (25 mg L−1).
aeruginosa cells mL−1 and mixed during 10 min, and finally the membrane pieces were placed in the culture vessel.
Membrane pieces were blocked with 5% wt/vol milk in TBST, cut into small pieces and incubated over night at 4°C in 1 ml TBST and 1 ml antiserum.
For the evaluation of the biomaterial's surface, membrane pieces of 1 cm2 were submitted to scanning electron microscopy in a Quanta 200 3D Scanning Electron Microscope (Fei Company, Hillsboro, USA).
In order to mimic a more representative biofouling formation, membrane pieces were submerged in a lab-scale MBR and processed in three different ways (1 h adhesion, 1 h adhesion followed by 24 h further incubation in Ringer, or in the MBR).
Membrane pieces were washed thrice with TBST and antibodies were eluted by adding 200 μl 100 mM Glycine-HCl (pH 2.5) and vortexing for 1 min. Purified antibodies were then transferred to fresh tubes and neutralised immediately by adding 10 μl 1 M Tris-Base.
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