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Fish oocyte membrane permeability parameters are reported here for the first time.
Membrane permeability parameters, including loss of 260 nm absorbing materials, change in extracellular conductivity, and extracellular pH, were used to reflect the integrity of membrane.
It was not possible to estimate membrane permeability parameters for stage V oocytes using the methods employed in this study because stage V oocytes experienced the separation of outer oolemma membrane from inner vitelline during exposure to cryoprotectants.
The cell membrane permeability parameters, including the hydraulic conductivity (Lp), the CPA permeability and the reflection coefficients in the Kedem Katchalsky (K-K) model, and the Lp and ω in the two-parameter model were determined.
To determine membrane permeability parameters for human iPSCs, we adapted our calcein fluorescence quenching method (A.K. Fry, A.Z. Higgins, Cellular and Molecular Bioengineering 5 (2012) 287 298) for use with an automated plate reader.
More broadly, the consideration of the concentration dependence of membrane permeability parameters may be important for other cell types as well, especially for design of methods for equilibration with the highly concentrated solutions used for vitrification.
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A factorial design experiment was carried out to illustrate the effects of main factors involving membrane permeability and operating parameters (feed concentration, temperature and flow rate) on the VMD performance, and some interactions among the effects were shown to occur as well.
The same parameter values as in the Data Fitting to Determine the Membrane Permeability and Slip Parameter Section were used, although now U = 0.6193 m s−1, and the mean values of k and α were inputted.
Using the mean values of k and α determined in the Data Fitting to Determine the Membrane Permeability and Slip Parameter Section, this width is 2.047 µm, and so the total width of boundary layers is 2.05% of the membrane wall thickness.
We validate the model, together with the mean values of k and α determined in the Data Fitting to Determine the Membrane Permeability and Slip Parameter Section, by comparing the theoretical predictions of retentate and permeate flowrates against experimentally measured values for a new lumen inlet flowrate of 4.66 mL/min.
Measured data were applied to water transport and nucleation models to determine membrane permeability and IIF nucleation parameters.
More suggestions(11)
membrane structure parameters
membrane stack parameters
membrane inflow parameters
membrane preparation parameters
membrane vibration parameters
membrane interaction parameters
membrane fabrication parameters
membrane system parameters
membrane charge parameters
membrane process parameters
membrane performance parameters
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