A Sytox permeability assay was performed in the wild type (BY4743) and ARL1 strain after overnight growth in YPD with COS-5.44 to assess cell membrane permeability [ 37] see Methods.
Parallel Artificial Membrane Permeability Assay (PAMPA) is a method to screen drug candidates for membrane permeability.
In vitro methods (passive artificial membrane permeability models, cellular monolayer models) are useful, although predictivity remains limited as the models cannot completely mimic the complexity of a dynamic in vivo system.
Therefore, alternative methods to determine osmotic membrane permeability of these cells are warranted.
Herein, a new approach for intracellular sensing of ROS by using horseradish peroxidase-encapsulated hollow silica nanospheres (designated HRP@HSNs), with satisfactory catalytic activity, cell membrane permeability, and biocompatibility, was prepared via a microemulsion method.
It was not possible to estimate membrane permeability parameters for stage V oocytes using the methods employed in this study because stage V oocytes experienced the separation of outer oolemma membrane from inner vitelline during exposure to cryoprotectants.
Computational models and physicochemical methods such as the Parallel Artificial Membrane Permeability Assay (PAMPA-BBB) offer high-throughput screening capability at early stages of drug discovery, but are only able to predict passive permeation [ 4, 5].
On the other hand, TBARS expressed as MDA equivalents and membrane permeability as relative leakage rate were determined according to the method of Xing et al. [ 17].
Membrane permeability, expressed as relative electrolyte leakage rate, was determined by the method of Jiang and Chen [22].
Membrane permeability, expressed by relative leakage rate, was determined according to the method of Duan et al. [6].
