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Membrane order was evaluated using the fluorescent probe 6-dodecanoyl-2-dimethylaminonapthalene (Laurdan) (Roche, UK).
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When regions of low membrane order are excluded, the higher order of the flagellar membrane can clearly be seen (Fig. 4E).
The effects of the Rvs161/167 BAR domain on membrane fluidity and lipid order are dependent on lipid composition.
Model membrane order parameter calculations were from Garnier-Lhomme et al. [34].
Interestingly, the change in membrane order, previously thought to be imperative for high rates of hydrolysis, was not required when membrane lipids were oxidized.
Roux et al. [30] showed that the force required to form tubes from a liquid ordered membrane is 1.7 times stronger than for a liquid disordered membrane.
In this study, SPG membrane emulsification in the absence of shear flow at the membrane surface was investigated in order to optimize the emulsification process and to gain deep insight into the droplet formation behavior.
To improve solubilization yields, sonication of the purified membrane fraction was performed in order to homogenize the suspension and to obtain smaller vesicles, more accessible to detergents.
A large scale expression and subsequent membrane preparation was made in order to analyse whether overexpression affected membrane localisation of heterologous protein.
The affinity order of the membrane was Pb > Cu > Cd, with the uptake of the ions per gram of membrane: 0.59, 0.47 and 0.33 m mol/g, respectively.
The results of MFP revealed that the adhesive force between the foulant (BSA) and membrane surface was at least an order of magnitude lower for the modified membranes when compared to virgin membranes.
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