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By incorporating unnatural amino acid photochemical and new click-chemical probes into the intact receptor expressed in the native membrane of live cells, 44 intermolecular spatial constraints have been derived for the ligand-receptor interaction.
EthD-1 is excluded by the intact plasma membrane of live cells.
Lectin-coated QDs have been used to label gram-positive bacteria and a single QD can be tracked for several minutes as it diffuses into the membrane of live cells and moves within the cytosol [77].
Thus all GFP-H-RasG12V-Gal-1 complexes exhibit relatively stable interactions with the plasma membrane of live cells.
Using a sensitive Fluorescence Resonance Energy Transfer (FRET) imaging method, we investigated the formation of CCR5 and CXCR4 heterodimers on the plasma membrane of live cells.
Here we investigated the effect of three major Cavβ types, β1b, β2d and β3, on the structure of Cav1.2 in the plasma membrane of live cells.
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Itoh, R.E. et al. Activation of rac and cdc42 video imaged by fluorescent resonance energy transfer-based single-molecule probes in the membrane of living cells.
We have investigated the origin of the dielectric response of the plasma membrane of living yeast cells (Saccharomyces cerevisiae) by using radiofrequency dielectric spectroscopy.
Here, we present a novel method to allow antibodies to penetrate the cellular membrane of living cells without affecting cell viability.
Recent developments in microscopy have led to revolutionary advances in our knowledge of the spatiotemporal dynamics of proteins in the plasma membrane of living cells and of heterogeneity of the plasma membrane.
Acridine orange stains both the dead and viable cells, whereas ethidium bromide selectively stains the dead cells as it cannot pass through the intact membrane of living cells.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com