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Therefore, for prokaryotes, the partitioning parameter is more favorable for membrane localization when compared to vertebrates.
CASTAWAY, an RLCK required for organ abscission, showed reduced plasma membrane localization when a G2A mutation was introduced for the putative myristoylation site.
Our in vitro data indicated that S100A14 and S100A16 are dependent on each other for their membrane localization; when either protein was knocked down, the other protein was not expressed on the membrane although there was no reduction in its mRNA expression level.
To understand the molecular consequences of the human SNP resulting in arginine (R) to threonine (T) exchange at position 345 in the amino acid chain of PRG‐1, we established HEK cell lines with stable expression of the mouse homolog of this SNP, PRG‐1R346T, and found similar membrane localization when compared to wild‐type PRG‐1 (wtPRG‐1, Fig 1A).
Similar(56)
Thus, most aspects of border cell differentiation and membrane localization were unchanged when bbg levels were reduced.
In the current quantitation, the authors compared apical to cytoplasmic Rho1; however, it appears that the lateral membrane localization of Rho1 increases when Skittles is expressed.
Both miRFP670nano internal fusions demonstrated perfect membrane localization, co-localizing with mVenus containing a CAAX-motif for membrane targeting.
Therefore, all parameters should be weighted accordingly when calculating the membrane localization score.
Deletion of the SH3 domain is sufficient to render the murine c-abl proto-oncogene product transforming when myristylated N-terminal membrane localization sequences are also present.
Although it was not our initial focus, we considered the β-catenin localization when absent from the cell membrane.
In addition to membrane localization, a small percentage of the EGR receptors were localized to the cytoplasm basally when co-expressed with HER2 in HEK293 cells.
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