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Indeed, some membrane labeling was detected, in scarce fields.
In the case of RFP-PH, the intensity of the plasma membrane labeling was proportionally increased with the dose of the injected protein up to 330 µM (concentration in pipette).
Cytoplasmic staining of any intensity without membrane labeling was considered intensity 1 [ 17].
Interestingly, at 14 d loss of epidermal membrane labeling was strongly decelerated in comparison to controls (compare Figure 6B to Figure 1E and Figure 6C to Figure 1B).
Surprisingly, although laboratory-reared flies can live for several months, loss of epidermal membrane labeling was apparent within a few days of eclosion and progressed steadily so that very little epidermal membrane labeling remained by six weeks.
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Clear concentration-dependent membrane labelling was seen up to 50 nM in CHO-β1 cells.
Similar to the paleocortex, plasma membrane labelling was dominant in the superficial layer at P3 (69%%) and with maturation the transport vesicle-associated labelling increased (P3: 31 %; P12: 41.8 %).
Vacuole membrane labelling was also detected in planta via immunoelectron microscopy using a CNGC20-antiserum on cryopreserved ultrathin sections of roots.
Clear membrane labelling could be seen in CHO-ssβ1 cells when imaged using the 488 nm (green) channel. 2 nM BODIPY-TMR-CGP (circa 3× KD concentration at β1-adrenoceptor site 1) was able to bind to CHO-ssβ1 cells and clear membrane labelling was observed in the 543 nm (red) channel.
Accumulation of newly labeled lactase in brush-border membranes after intraperitoneal [35S]methionine labeling was similar in both groups at 3 h.
Electron microscopy data of the intracellular distribution GSK3β in neurons revealed that GSK3β is expressed in the mitochondrial membranes, and robust GSK3β labeling was found on ribosomes and the rough endoplasmic reticulum.
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