In MD GLE retinas, BrdU labeling persisted until PND5; we observed no BrdU-IR cells on PND7.
(b) EM image showing plasma membrane labeling in a HEK293T cell transfected with HRP-TM (asterisk).
Indeed, some membrane labeling was detected, in scarce fields.
Membrane labeling experiments followed those described previously [20].
Cells were labeled with membrane labeling dye PKH26 (Sigma-Aldrich), according to the manufacturer's instructions.
Most vacuoles incorporated FM dye immediately upon surface membrane labeling, suggesting that these structures were pre-existing and continuous with the plasma membrane at the time of labeling.
After the PPN stimulation, these parameters immediately returned despite that the membrane hyperpolarization persisted.
The membrane hyperpolarization persisted for 10 20 s even after termination of the stimulation.
The first and third rows correspond to cytosolic membrane (labeled in green) and polymerized F-actin (labeled in red), respectively.
For lipid quantification membrane labeled rhodamine fluorescence was measured in a BioTek Synergy HT plate reader.
