As the apical membrane labelling, observed in the adjacent normal pancreatic tissue, is preserved in almost all PDACs, we might speculate that ADAM9 function could at least partly be maintained in the tumour cells.
Note that the Jen1-KR-GFP presents defects in ER exit upon synthesis (ER labeling observed throughout the experiment) but still manages to reach the plasma membrane, and is not associated with the vacuole.
In contrast to the inclusion labeling observed with anti-CPn0186 anoneofies, none of the 7 sera stained the inclusion membrane.
While in our study, we did not perform GFAP staining to determine whether astroglial cells had FITC-EDC membrane labeling, we did observe some cells in the hippocampus with typical glia morphology that displayed FITC-EDC membrane labeling.
DbH and VMAT2 labeling appeared in a consistent pattern of dense core vesicles, with VMAT2 labels observed associated with vesicular membranes and DbH labeling seen at either membrane or lumen of the vesicle.
Using this method, we obtained similar results to those observed for membrane labeling: the mean fraction of labeled CySCs increased as a function of time (Supplementary Fig S2E and F) and the number of testes harboring labeled clones decreased over time (Supplementary Fig S2G and H).
The overall number of self-membrane contacts observed during the focal membrane labeling assay tended to be lower after the cytochalasin D treatment but was statistically insignificant from the non-treated controls.
Our observations of T-tubules in rat atrial myocytes are consistent with those previously observed using electron microscopy and fluorescent membrane labeling.
Indeed, some membrane labeling was detected, in scarce fields.
Membrane labeling experiments followed those described previously [20].
