Exact(8)
Particularly, it is only the superficial colorimetric signal that could be used for quantitation, while the deep signal in the membrane is lost.
PI is an impermeable dye which stains the nucleus only when the integrity of the cell membrane is lost, which can be related to the late stage of apoptosis [21, 50].
During this time, the smooth appearance of the plasma membrane is lost and the cell becomes irregular in structure.
Eventually these cells contain almost all DNA and very little cytoplasm, as suggested in these scans, where a clear demarcation between cellular and nuclear membrane is lost.
Although nearly the same amount of membrane is lost in C739 mutant cells over time, the rate of membrane loss is decreased by more than 3-fold (Fig. 7 B, P < 0.01).
Accordingly, when the balance of activity between pro- and anti-apoptotic members is upset, the permeability of mitochondrial membrane is lost and mitochondrial reactive oxygen species (ROS) is induced.
Similar(52)
We suggest that the luminal progenitors, which normally replenish the membrane, are lost upon extended exposure to 5-HT, resulting in a loss of the regenerative capacity of the epithelium.
A muscle structure that attaches thin filaments to the muscle membrane was lost in dys-1 mutants.
Time of lysis was indicated by a rise in intracellular calcium prior to lysis, followed by a drop in fluorescence when integrity of the plasma membrane was lost.
Drosophila eggs do not develop a serosa, as this extraembryonic membrane was lost in the Schizophoran flies (Schmidt-Ott, 2000; Rafiqi et al., 2008).
When cells occupy the lumens of MCF-10A acini, cell survival cues provided by integrin contacts with the basement membrane are lost.
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