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In all cases a fast-electrosynthethized polypyrrole (PPy) membrane is generated for this purpose.

The tethered bilayer lipid membrane is generated by substitution of protein-bound detergent molecules with lipids using in-situ dialysis or adsorption.

The ΔpH needed for Na+ transport over the tonoplast membrane is generated by V-H+-ATPase and V-H+-PPase (Apse et al. 1999; Gaxiola et al. 2007).

A consequence of this is that the poles of the cells are not round but flat, and that a relative sharply curved membrane is generated at the transition from a pole to a lateral wall (see model in Figure 6E).

The proton gradient across the cristae membrane is generated by three large membrane protein complexes of the respiratory chain in the cristae, known as complex I (NADH/ubiquinone oxidoreductase), III (cytochrome c reductase) and IV (cytochrome c oxidase) (Fig.  2).

In particular, even though up to 50% of the vacuole membrane is generated de novo in a given cell (Catlett and Weisman, 2000), actual vacuole copy number is likely not strongly affected by de novo vacuole biogenesis, consistent with the most widely accepted model of vacuole biogenesis (Wickner, 2002).

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Asymmetry between the inside and the outside of the liposome membrane was generated through the enzymatic action of phospholipase D, where lipid head groups in the outer membrane leaflet, i.e. exposed to the enzyme, were hydrolyzed.

To more precisely identify the Bves-Bves interaction region in the C-terminus, a SPOTs blot membrane was generated by SIGMA-GENOSIS (Woodland, TX).

A marker for the chloroplast envelope inner membrane was generated by fusing A. thaliana plastidic nucleotide transporter 2 (Nto2) thethe N-terminus of the monomeric red fluorescent protein (RFP) variant, mCherry.

Noticeably, small vesicles positive for the autophagic membrane protein ATG9 were also generated in the reaction, but in this case vesicles formed independent of PI3K or COPII, suggesting that this potential source of autophagosomal membrane was generated in a process distinct from that of the LC3 lipidation active vesicles.

At the same time, porous polymer membranes were generated by removing the silica component with hydrofluoric acid.

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