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This motion of the basilar membrane is detected by the inner hair cells.
In the case of methanol and ethanol separation a lower separation capability of the different PDMS membrane is detected than a flash distillation.
The retrotranslocation of fluorescence-labeled substrates from the lumenal to the cytosolic side of the membrane is detected because the cytosol also contains fluorescence-quenching agents.
We show that the lysosome- associated membrane protein 1 (LAMP1), normally resident on the internal leaflet of the lysosomal membrane, is detected at the cell surface following in vitro treatment of mouse epiphyseal chondrocytes with ionomycin, and that ionomycin treatment induces release of aggrecan-degrading hydrolases in culture.
Bruch's membrane is detected by searching the pixel with the biggest gradient value above the retinal pigment epithelium (RPE) and the choroidal-scleral interface is delineated by finding the shortest path of the graph formed by valley pixels using Dijkstra's algorithm.
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Secondary antibodies binding on the membrane were detected by chemiluminescence ECL detection system (Amersham-Pharmacia Biotech Inc., Piscataway, NJ, USA) using Fujifilm LAS-3000 LumImageent ImAnalyzeryzer (Fujifilm Corporation, Tokyo, Japan).
The bound antibody on the membrane was detected using an enhanced chemiluminescence detection system according to the manufacturer's manual (Amersham Pharmacia Biotech, Amersham, UK).
Proteins on the membrane were detected using a West pico chemiluminescence detection kit and quantified with a ChemiDoc (BioRad) digital image system using a QuantityOne software.
The biotin-labeled molecules in the membrane were detected using a chemiluminescent nucleic acid detection module (Pierce Biotechnology).
Protein binding on the membrane was detected by the enhanced chemiluminescence (ECL) detection system (Pierce, Rockford, IL) according to the manufacturer's instructions.
The presence of the functional groups of PDDA on the membrane was detected by FTIR.
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