Local background was calculated from a cell-free region one cell diameter away and subtracted from all membrane intensity measurements prior to calculation of 402/490-nm excitation ratios that were fitted to calibration curves to derive cortical pH.
These changes were reflected in the measurements of the mean membrane intensity and actin membrane/cytosol ratio (Fig. 8B).
When samples of substandard insecticide formulations are diluted as per the pre-determined dilutions, the aliquots are expected to contain less than the quantity specified above, and would result in the appearance of two purple lines, with one of them at the sample line region of the membrane, the intensity of which would be inversely proportionate to the concentration of the test analyte.
For meaningful comparison of intensities across different membranes, the intensity of each spot on a membrane was represented as relative intensity RI = (Xi-B /G, where Xi-B /Ghe intensity of the ith point of membrane, and B and G are the baseline intensities of empty spot and GST (5 ng) respectively.
Primary categorical analysis was carried out as follows: positivity for CK5/6 and EGFR was set as ≥10% of cells staining, and for HER2, tumor cores that showed membrane-staining intensity of 2 or 3 were considered positive.
A) For the whole genome DNA array membrane (Eurogentec membranes), the average intensity of the empty spots was subtracted from each spot intensity value.
It was demonstrated that from a life cycle perspective two hurdles exist to further development of the ODN-SWRO process: module design of FO membranes and cleaning intensity of the FO membranes.
It is noteworthy to mention that, due to a very low amount of lipophilic additive in the matrix of the membranes and high intensity of C = O, C = C absorption bands which overlapped with the C – Br stretchings, it was not possible to deduce the presence of lipophilic additive individually from the presented FTIR spectra.
β-cat staining was manually quantified by drawing a five-pixel thick line along the membranes measuring integrated intensity of sum-projected z-stacks.
The amount of plasmid used for the soluble protein was constant, whereas the amount of plasmid for the membrane protein was increased by a factor 3. Intensity of membrane protein expressed alone was set to 1. Using this method we showed that using a 1 1 TMD Sol plasmid ratio increases the signal by a factor 1.9 (in theory a 2 fold increase is expected).
