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Results showed that Mz-ChA-1 and QBC939 cells treated with matrine for 24 h presented extensive organelle and cell swelling and cytoplasmic vacuolation; these cells lost the plasma membrane integrity when treated with matrine for 48 h (Figure 1c).
The cross-linked SSBS membranes were found to absorb less water, showed higher resistance to thermal degradation and, when not highly cross-linked, showed better membrane integrity when immersed in water for extended periods.
PI fluorescence greatly increases upon binding to nucleic acids and so it can be used as a marker for membrane integrity when added to the extracellular milieu.
Significant deleterious effects on semen quality were detected for membrane integrity when semen had been stored for 2 days after sampling.
Alterations of the cytoskeletal proteins that connect the bilayer and the spectrin network, impact on particularly the erythrocyte membrane integrity, when encountering shear stresses [ 5].
Although they found a negative effect on membrane integrity when overexpressing Fst in E. faecalis cells, the mechanism of action of Fst is obviously different from antimicrobial peptides.
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The lactate dehydrogenase (LDH) leakage from human macrophages exposed to various concentrations of A. actinomycetemcomitans leukotoxin for 60 min indicated that a dose-dependent disruption of the membrane integrity occurred when leukotoxin concentrations were ≥1 ng/ml.
SG enters cells with compromised membrane integrity and fluoresces when interacting with double stranded nucleic acids.
Annexin V is a 36 kDa Ca2+ dependent phospholipid-binding protein that has a high affinity for PS and binds to cells when PS is exposed to the external cellular environment, which occurs when membrane integrity is affected in early phase of apoptosis [16] [17].
This was perhaps not surprising, as NR indicates when membrane integrity is lost, late in the apoptotic process.
However, this loss does not occur until late in the apoptotic process, when membrane integrity is compromised.
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