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The results indicated a significant interaction between EcN and EcA on growth performance and jejunal mucosal membrane integrity, morphology, immune parameters and the antioxidant capacity in 21-day-weaned piglets (P<0.05).
In order to evaluate the ability of both plant extracts to reduce oxidative stress in H2O2-treated cells, co-treatment and pre-treatment experiments were done and results are presented in Figure 2. Cell viability, membrane integrity, morphology and cell cycle were investigated.
Spermatozoa with damaged chromatin appear normal in terms of their membrane integrity, morphology and motility, but their use would lead to post-fertilization embryo failure [ 5].
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At 4 micrograms/ml, bleomycin has no effect on cell membrane integrity or morphology but results in a significant increase in intracellular oxidation.
The apparent cell survival immediately after thawing, evaluated from cell membrane integrity and morphology, was low, typical to the tested freezing conditions and relatively similar in all the samples with no discernable trend.
Single Layer Centrifugation (SLC) is a colloid centrifugation technique designed to select spermatozoa with good motility, membrane integrity, normal morphology and intact chromatin from the rest of the ejaculate [ 2].
Fresh semen was used for initial analysis and plasma membrane integrity and sperm morphology were also determined.
However, the cells maintained their plasma membrane integrity, and apoptotic morphology was only observed in a minority of the cells.
We have adapted the Hoechst/PI assay in microtitre plate format for microscopic identification and quantification of membrane integrity and nuclear morphology.
Motility was assessed subjectively and membrane integrity as well as morphology evaluated after eosin-nigrosin staining.
Filamins stabilize F-actin networks in the cell and link them to cellular membranes by binding to transmembrane receptors or ion channels, thereby regulating cell morphology, membrane integrity and cell locomotion [ 5].
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