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Nickel, W. et al. Content mixing and membrane integrity during membrane fusion driven by pairing of isolated v-SNAREs and t-SNAREs.
This, and the loss of cell membrane integrity during cell death, make La accessible to DAB4 binding and means that DAB4 preferentially binds to dead tumour cells[10].
This hypothesis is also supported by the presence of propidium iodide positive cells around the wounded area, which is indicative of loss of plasma membrane integrity during the wounding process (Figure S1B).
Propidium iodide was included in the imaging buffer to monitor plasma membrane integrity during the response.
Moreover, KDO assay showed outer membrane integrity during secretome concentration (data not shown).
10.7554/eLife.00459.017 Figure 4. ARTs protect plasma membrane integrity during heat stress.
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We demonstrated forward osmosis (FO) membrane integrity loss during gypsum scaling with the presence of membrane spacer.
As cell membrane integrity is lost during the late phase of apoptosis, cytoplasmic La becomes accessible to binding by specific mAb, which itself in turn becomes crosslinked in the dying cell by TG2 [14].
As cell membrane integrity is lost during the late phase of apoptosis, cytoplasmic La becomes accessible to binding by specific mAb, which in turn becomes crosslinked in the dying cell by TG2 so that its levels are higher than in dead cells of the counterpart primary cell type [29].
In contrast to necrosis, membrane integrity is maintained during apoptosis precluding staining of DNA by the membrane impermeable dye propidium iodide (PI).
The French press generates a physical shearing force to skin mitochondria of the outer membrane without disrupting membrane integrity which occurs during osmotic swelling or detergent treatments (Hoppel et al., 1998).
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