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Over half of the responding genes were associated with membrane integrity and function, however, neurological and muscular function was also affected.
The up-regulation of genes encoding HSP proteins and E3 ubiquitin ligases suggests that protein misfolding occurs under ethanol stress, possibly affecting proteins that help maintain plasma membrane integrity and function.
The changes in cell membrane fatty acid composition profiles and synthesis of stress proteins, especially small heat shock proteins were required for active cell response to maintain membrane integrity and function under stress conditions.
Physiologically, hydrocarbon accumulation in cell membrane causes loss of membrane integrity and function, which ultimately leads to cell death [ 7].
Plant membrane integrity and function, determined by structure and fluidity, are significantly affected by lipid composition, particularly the degree of fatty acid desaturation [ 23].
Membrane integrity and function, determined by structure and fluidity, are largely affected by lipid composition and the degree of fatty acid desaturation in plants [ 23].
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The linkage between ECM and the cytoskeleton through DGC is critical for the membrane integrity and functions of skeletal muscles.
In addition, cell membrane integrity and mitochondrial function could be assessed after addition of specific respiration inhibitors or stimulators.
Fatty acid unsaturation is thought to reduce the propensity of cellular membranes to undergo freezing-induced, non-bilayer phase formation, thus enhancing membrane integrity and cellular function during freezing [ 44].
In parallel to incubations for assessing NO production, cell viability after exposure to ZEN was measured using neutral red (3-amino-7-dimethylamino-2-methylphenazine hydrochloride, NR) upthee based on the method described by Borenfreund and Puerner [ 21] in order to evaluate membrane integrity and lysosomal function.
This could also be attributed to higher production of oxygen radicals, disintegration of membrane integrity and cytoskeletal functions as a result of treatment.
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