A total of 200 300 nuclei with clear boundaries appearing to have no loss of membrane integrity were identified for analysis from each tissue sample, overlapping nuclei were excluded.
A total of 100 200 nuclei with clear boundaries appearing to have no loss of membrane integrity were identified for analysis from each tissue sample.
Analyses of sperm motility by computer-assisted sperm analysis (CASA), sperm nuclear morphometry by computer-assisted sperm morphometry analysis (CASMA), membrane integrity by acridine orange-propidium iodide combination and sperm DNA fragmentation using the sperm chromatin dispersion test (SCD) were performed.
Flow cytometric analysis employing membrane integrity indicators like propidium iodide (PI) and thiazole orange (TO) indicated a greater sensitivity of Arthrobacter cells to cesium than to strontium.
The sperm characteristics assessed were motility (measured subjectively [SM] and by means of cell motion analysis (CASA)), membrane integrity (SYBR-14/PI), and capacitation status (chlortetracycline (CTC)/EthD-1).
Optimality of the chosen cryoprotectants was confirmed by cell viability (PI live/dead staining), cell proliferation (MTT assay), microstructure analysis (SEM), membrane integrity (confocal microscopy) and in vitro osteogenic differentiation (ALP assay, RT-PCR and histology) study carried out with post-thaw cryopreserved TECs.
Toxicity on gram-negative bacteria (Escherichia coli), gram-positive bacteria (Staphylococcus aureus and Staphylococcus epidermidis), and pathogenic yeast (Candida albicans) was evaluated by analysis of cell morphology, assessment of cell viability using the PrestoBlue assay, analysis of cell membrane integrity using the lactate dehydrogenase assay, and reactive oxygen species production.
Fresh semen was used for initial analysis and plasma membrane integrity and sperm morphology were also determined.
Flow cytometric analysis using the membrane integrity indicators demonstrated a significant reduction in the viable cell populations following exposure to the increasing concentrations of Cs+ or Sr2+ suggesting a dose dependent relationship (Fig. 3a, b).
Subsequent analysis of mitochondrial membrane integrity by fluorescent JC-1 dye revealed extensive depolarization of the mitochondrial membrane in human neurons exposed to Tat and morphine.
