Studying differentiation, we cultured tissue explants and cells from passage one on hanging membranes either with access to medium from both basolateral and apical side or in an air-liquid interface with access to medium only from the basolateral side.
Cells were grown on permeable membranes (6-well and 24-well Millicell hanging inserts, PET membrane, Millipore, Temocura, Canada) hanging in tissue culture plates and creating two compartments, a basolateral and an apical one.
In the mean time, identifying physical characteristics that correlate with protein expression might aid in selecting the "low hanging fruit of membrane proteins", or proteins that can be expressed to sufficient levels using an E. coli expression system.
Polyethylene terephthalate membrane (8 μm pore size) Millicell hanging cell culture inserts were purchased from Millipore.
The cell invasion assay was performed using a polyethylene terephthalate membrane (8 μm pore size) with Millicell hanging cell culture inserts and BD Matrigel.
A hanging curtain is replaced by an equivalent membrane, the equation of motion of which is derived.
Ms. Alford worked in her classroom last Tuesday, carefully hanging an animal-cell poster illustrating the plasma membrane and Golgi complex with rolled masking tape.
A new large-scale pilot hybrid treatment system of 53 m3/day was developed by combining 3 treatment methods: switched internal recycling flows to equalization tank (EQ); rotating hanging media bioreactor (RHMBR); and submerged flat sheet membrane bioreactor (SMBR).
A specifically designed pilot-scale hybrid wastewater treatment system integrating an innovative equalizing reactor (EQ), rotating hanging media bioreactor (RHMBR) and submerged flat sheet membrane bioreactor (SMBR) was evaluated for its effectiveness in practical, long-term, real-world applications.
Prostaspheres were cultured in Millicell hanging cell culture inserts with 1.0 µm PET transparent membranes (Millipore) on 6-well plates (Costar).
