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Infection of cells and subsequent formation of syncytia occur through membrane fusion mediated by the RSV fusion protein (RSV-F).
However, the detailed molecular mechanism of the membrane fusion mediated by the spike (S) protein of HCoV-229E remains elusive.
Moreover, purified drosophila ATL has been reconstituted into proteoliposomes, and signals of lipid mixing in the presence of magnesium and GTP provide direct evidence for membrane fusion mediated by ATL (Orso et al., 2009; Bian et al., 2011).
After entry into the cytoplasm through membrane fusion mediated by glycoproteins, the RNP is released from the virion and serves as the template with which the copackaged RdRp transcribes mRNAs from the viral genome in the RNP.
Membrane fusion mediated by coronavirus, similar to other viruses, is a multistep process.
For example, it has been suggested that glycosphingolipids in the target membrane play an important role in membrane fusion mediated by the HIV-1 envelope glycoproteins [74] [79].
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The currently evidence suggest this lipid likely participates in key processes by altering membrane architecture necessary for membrane fusion, mediating the penetration of membrane proteins, serving as a precursor for other important SV cycling lipids, or activating essential enzymes.
Membrane fusion is mediated by SNAREs (soluble N-ethylmaleimide-sensitive factor attachment receptor proteins).
As for many essential biological processes, intracellular membrane fusion is mediated by interactions among a series of evolutionarily conserved proteins.
Cellular membrane fusion is mediated by the SNARE family members, while viral membrane fusion is accomplished by three classes of viral fusion proteins.
Because, mitotic Kar5p expression is not toxic, we favor the hypothesis that outer membrane fusion, perhaps mediated by a pheromone-induced protein such as Prm3p, primes Kar5p for INM fusion.
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