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We show that basal membrane function can be substituted in vitro by extracellular matrix (ECM) proteins and that ES cells can be induced to form similar polarized rosettes that initiate lumenogenesis.
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Membrane protein function can be regulated by changes in membrane lipid composition [1] [8].
To further investigate the performance of the proposed algorithm for properly recovering also slowly varying phases, we constructed a phase object with the phase given by a continuous function that is not piecewise constant (Fig. 6a), namely the L-shaped membrane; this function can be obtained by the MATLAB expression 'membrane'membrane
Other induced changes that impact membrane structure and function can be important.
Any perturbation to mitochondrial function will affect the membrane potential and the integrity of mitochondrial function can be tested by the rate of mitochondrial membrane depolarization (collapse of the membrane potential) in living cells.
All results demonstrate that versatile PVDF membranes with multiple functions can be facilely designed by the efficient interfacial crosslinking strategy.
This approach has several advantages: it can yield increased multiplexing capacity, it can be extended to assays for secreted, cytoplasmic, and membrane proteins, and measurements of cellular functions can be integrated with the proteomics assays.
Co-operation between functions can be limited.
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