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For further outer-membrane fractionation, the pellet (total membrane fraction) was resuspended sterilized distilled water.
Our fractionation protocol aimed to enrich the presynaptic active zone membrane fraction as well as the PSD membrane fraction, while cytosolic fractions were not analyzed.
The crude membrane fraction was collected by ultracentrifugation at 180,000g for 1 h.
Neuregulin increased the p35-associated Cdk5 kinase activity in the membrane fraction of cultured C2C12 myotubes.
After centrifugation (10 min 13,400 × g) the supernatant contains the membrane fraction.
The protein concentration of the membrane fraction was measured by the Lowry method.
Next, the pellet containing the plasma membrane fraction was suspended in SHB buffer and collected for Western blot analysis.
Wg signaling generates a hyperphosphorylated form of Dsh, which is associated with a membrane fraction.
Both novel bands localize to the membrane fraction after differential centrifugation.
Moreover, a latent phosphatase activity was found predominantly in a sequestered membrane fraction of frog erythrocytes.
Two protein bands (40- and 21-kDa species) immunoreactive to PLA2 antibody were detected in the membrane fraction.
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