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Also temperature variation influences the transport process, which could be due to alterations of transporters efficiency induced by membrane fluidity modifications.
Cauda sperm from siRNA-treated rats were prepared for membrane fluidity assay using a slight modification of the previously described protocol [52], sperm were released into the capacitation medium containing 25 nM Yo-Pro-1 without BSA.
The presence of this modification affects membrane fluidity and influences antimicrobial peptide resistance.
Modifications of membrane fluidity were shown to occur at pressures above 100 MPa (Hauben et al.[1997]) and protein denaturation was observed at more than 400 MPa (Aertsen et al.[2009]).[2009]
To evidence possible modifications in membrane fluidity induced by penetratin interaction with lipids we used the fluorescent probe Laurdan.
Its mechanism could be the induction of water pores across the lipid bilayer, together with modification of the membrane fluidity [ 92].
Isoprenoids are found in all three domains of life and are involved in essential processes such as electron transport, post-translational modification, regulation of membrane fluidity, and cytoskeleton assembly.
High temperature causes modifications in membrane functions, associated with alteration of membrane fluidity.
Therefore, the antimalarial activity of MeSter would directly depend on a profound alteration of membrane fluidity.
The change of membrane fluidity may affect membrane-associated functions, such as transportation of nutrients.
Active proliferating cancer cells present not only quantitative changes in de novo lipid biosynthesis but also modifications of lipid membrane composition affecting membrane fluidity, signal transduction and gene expression [3], [4].
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