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A longitudinal tension appears to be required to pull apart the membranes and allow membrane fission during membrane trafficking in yeast, vertebrate and mammalian cells [25] [27].
Indeed, by virtue of its interactions with Dyn2 (this study), cytosolic proteins involved in membrane morphogenesis such as αCOP [4], cytoskeletal proteins and molecular motors [2], [3], [12], [49], [50], Ndel1 would be a candidate of choice to integrate cytoskeleton dynamics and membrane fission during membrane trafficking.
Both the forespore and mother cell SpoIIIE subcomplexes are required to maintain septal membrane fission during DNA translocation.
Dynamin is a GTPase protein that is essential for membrane fission during clathrin-mediated endocytosis in eukaryotic cells.
SpoIIIE is a membrane-anchored DNA translocase that localizes to the septal midpoint to mediate chromosome translocation and membrane fission during Bacillus subtilis sporulation.
Our data suggest that SpoIIIE assembles a coaxially paired channel for each chromosome arm comprised of one hexamer in each cell to maintain membrane fission during DNA translocation.
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Furthermore, the observation that the separated membranes before degradation were converted to continuous membranes after degradation suggests that membrane fission is reversible during DNA translocation and depends on the integrity of the paired channel.
Dynamin-like protein 1 (DLP1), a member of the large GTPase family, promotes the maintenance of peroxisomal and mitochondrial morphology, especially during membrane fission (Ishihara et al., 2009; Tanaka et al., 2006; Waterham et al., 2007).
During membrane fission, molecular motors are provided with a local energy source.
This was a useful model to explain the mechanism underlying the generation of PtdOH during CtBP1/BARS-induced membrane fission.
Formation of vacuolar sheets and foamy membrane structures in stage 4 presumably needs membrane fission and fusion, and eventually vesiculation of the vacuole during stage 5 necessitates membrane fission.
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