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The solution was diluted to 40.0 ml with 0.15 M acetate buffer pH 5.0 and membrane filtered (0.22 μm).
Culture supernatants were collected 24 hours later from the bottom chamber (facing the basolateral membrane), filtered through a 0.2 µm filter (Nalgene) and used to activate MoDCs.
The crude residue was solubilized in water (1 mL) with sonication and was membrane filtered.
The MorHap-BSA conjugate was membrane filtered and the protein recovery was determined by BCA assay.
The composite samples (composed of 4 10 well samples) were membrane filtered and cultured for Aeromonas as described above.
The BSA- PEG 2-MorHap conjugate (MorHap-BSA- PEG 2-MorHap filtered and the protein concentration was measured BSA- PEG 2-MorHap
Similar(48)
Membrane filters 0.45 μm were purchased from Agilent.
The extract was filtered through a 0.45 μm membrane filter.
All the samples were filtered through 0.45 mm membrane filter.
The samples were filtered through 0.45-μm Millipore membrane filters.
All samples were filtered through 0.22 μm polycarbonate membrane filter.
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