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The morphology and compositions of colloid specimens on the 0.2 μm membrane filter were analyzed by scanning electron microscopy (SEM, HITACHI TD-1000, Hitachi Ltd., Tokyo, Japan) with energy-dispersive X-ray spectrometry (EDS, HORIBA EMAX ENERGY EX-250).
Briefly, the inserts in the membrane filter were coated with Matrigel on the upper surface.
Thermo Slide-A-Lyzer® 10K cut off dialysis cassettes and 0.22 μm cutoff membrane filter were purchased from Fisher Inc.
Samples were dissolved in acetone (5% v/v) and after filtration through a 0.45 μm PTFE membrane filter were injected onto Merck KGaA (Darmstadt, Germany) LiChrospher 100 RP-18e 5 μm (250 mm × 4 mm) column under gradient condition [ 23].
After 24 h of incubation, cells remaining on the upper surface of the membrane were removed with a cotton swab and cells that invaded through the membrane filter were fixed with 100% methanol, stained by hematoxylin and eosin, and photographed by soft BioLife DP under a microscope (Olympus BX40 with a DP70 digital camera, Tokyo, Japan).
After 24 h of incubation, cells remaining on the upper surface of the membrane were removed with a cotton swab, and cells that migrated through the membrane filter were fixed with 4% paraformaldehyde, stained with 0.1% crystal violet, and photographed under a microscope (Olympus BX40 with a DP70 digital camera).
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A 0.4 μm microfiltration membrane filter was used to filtered the aqueous samples.
Millipore FHUP Fluoropore™ Membrane Filter was bought from EMD Millipore Corporation (Billerica, MA, United States).
A 0.45-μm membrane filter was used to remove unwanted materials from collected water samples.
With the present setup the argument of the basilar membrane filter is independent of c.
A parametric view of the modulus of the basilar membrane filter is shown in Figures 7 and 8.
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