Sentence examples for membrane filter units from inspiring English sources

Cell-free culture fluids for Vp extracellular factor activity assays were prepared by centrifugation at 5000 g for 10 minutes and filtration of the fluids through 0.2 µm-pore-size membrane filter units (Nalgene Labware Division, Nalge/Sybron Corp).

The solution was then filtered through disposable membrane filter units (DISMIC-13JP, ADVANTEC), evaporated, and stored at −80 °C until use.

Four μL of the amplified products were visualized on 1.5% agarose minigels and the remainder was purified using 30,000 NMWL low-binding, regenerated cellulose membrane filter units (Millipore).

The bacterial culture was centrifuged at 4000 g for 15 minutes at 4°C and the supernatant collected and filtered through a 0.22 µm pore size syringe membrane filter unit (Millipore).

The supernatant was filtered through 0.22  μm syringe driven membrane filter unit and 50  μL of the filtrate was injected onto the HPLC system.

Cell medium with nascent HDL was collected, filtered through a 0.45 μm PVDF membrane filter unit (EMD Millipore), reduced in volume 20× from 30 to 1.5 ml using an Amicon Ultracel-10K centrifugal filter (EMD Millipore) and stored at 4°C for further analysis.

The recovered supernatant, that contained the intracellular crude extract, was filtered through a PVDF membrane (Millex GV filter unit 0.22-μm, Millipore) and then quantified by the Bradford assay [ 24] using a standard curve of bovine serum albumin (BSA).

Following treatment, Hanks' Balanced Salt Solution was collected and centrifuged at 12,000 g for 10 min at ambient temperature and the supernatant was concentrated ∼50-fold using Amicon Ultra-0.5 Centrifugal Filter Units (Ultracel-30 membrane; Millipore, Watford, UK).

Mobile phase was filtered through a 0.2 mm membrane filter (Phenomenex, USA) using vacuum filtration unit (Phenomenex, USA) and was degassed in an ultrasonic cleaner (Cole-Parmer, Chicago, IL, USA) and delivered at a flow rate of 1 mL/min.

Amicon Ultra-15 centrifugal filter units with Ultracel-30 membrane (30K centrifugal filters) of 5 mL capacity were obtained from Millipore (Merck, Germany).

Supernatants were collected after centrifugation at 1,400 g for 5 min and up-concentrated using Amicon Ultra-0.5 Centrifugal Filter units with Ultracel-50 membrane (Millipore, Norway).