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The bacterial culture was centrifuged at 4000 g for 15 minutes at 4°C and the supernatant collected and filtered through a 0.22 µm pore size syringe membrane filter unit (Millipore).
The supernatant was filtered through 0.22 μm syringe driven membrane filter unit and 50 μL of the filtrate was injected onto the HPLC system.
Cell medium with nascent HDL was collected, filtered through a 0.45 μm PVDF membrane filter unit (EMD Millipore), reduced in volume 20× from 30 to 1.5 ml using an Amicon Ultracel-10K centrifugal filter (EMD Millipore) and stored at 4°C for further analysis.
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Cell-free culture fluids for Vp extracellular factor activity assays were prepared by centrifugation at 5000 g for 10 minutes and filtration of the fluids through 0.2 µm-pore-size membrane filter units (Nalgene Labware Division, Nalge/Sybron Corp).
The solution was then filtered through disposable membrane filter units (DISMIC-13JP, ADVANTEC), evaporated, and stored at −80 °C until use.
Four μL of the amplified products were visualized on 1.5% agarose minigels and the remainder was purified using 30,000 NMWL low-binding, regenerated cellulose membrane filter units (Millipore).
The recovered supernatant, that contained the intracellular crude extract, was filtered through a PVDF membrane (Millex GV filter unit 0.22-μm, Millipore) and then quantified by the Bradford assay [ 24] using a standard curve of bovine serum albumin (BSA).
The groundwater passed through the membranes in the filter unit under differential hydraulic pressures between the sampling intervals and the pressure regulators in order to avoid abrupt changes in the hydraulic pressure in each borehole: 1.7 and 1.4 MPa in 09MI20 and 2.7 and 2.4 MPa in10MI26.
The sample was then filter sterilized with a PES membrane, 0.22 μm Millipore filter unit (Millipore, Etobicoke, ON) into a 2 ml microcentrifuge tube on ice and stored at -80°C.
This final dilution was filtered through a 0.2-µm-membrane filter (Ultrafree®-MC filter unit, Millipore Corporation, Bedford, MA, USA) and stored at −75°C until HPLC-ED analysis.
The groundwater passed through a 0.2 μm membrane (ADVANTECH, Tokyo, Japan) placed in the filter unit.
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CEO of Professional Science Editing for Scientists @ prosciediting.com